StrokeNet RCC28 Mid-America Regional Coordinating Center (MARCC) at Washington University School of Medicine (WU) and affiliated Barnes-Jewish Hospital (BJH), in collaboration with six other high-volume tertiary care Stroke Centers spanning the Midwestern and Southern states of MO, KS, IL, TN, AK: St. Luke’s Hospital in Kansas City, MO; The Order of St. Francis Medical Center (OSF) in Peoria, IL; University of Tennessee Health Sciences in Memphis, TN; Cox Medical Center in Springfield, MO. Each of these medical centers is a tertiary care hub with large networks of referring hospitals, and have extensive experience participating in multi-center stroke trials. Drawing from both rural and urban settings, the hospitals of RCC 28 MARCC admit almost 7,000 stroke patients per year, including patients from diverse racial backgrounds and socioeconomic strata. Moreover, the collective group of stroke clinicians, investigators, and scientists that will participate in MARCC have diverse expertise that will permit the implementation of stroke trials across the translational and treatment spectrum. We look forward to expanding our network and patient enrollment further in the next StrokeNet cycle to continue to meet the joint goals of this network.